Unraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach.
Identifieur interne : 000009 ( Main/Exploration ); précédent : 000008; suivant : 000010Unraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach.
Auteurs : Evandro Silva [Brésil] ; José Perez Da Graça [Brésil] ; Carla Porto [Brésil] ; Rodolpho Martin Do Prado [Brésil] ; Clara Beatriz Hoffmann-Campo [Brésil] ; Mauricio Conrado Meyer [Brésil] ; Estela De Oliveira Nunes [Brésil] ; Eduardo Jorge Pilau [Brésil]Source :
- Scientific reports [ 2045-2322 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Feuilles de plante, Maladies des plantes, Soja.
- métabolisme : Feuilles de plante, Soja.
- physiologie : Phakopsora pachyrhizi.
- Métabolomique, Spectrométrie de masse.
English descriptors
- KwdEn :
- MESH :
- metabolism : Plant Leaves, Soybeans.
- microbiology : Plant Diseases, Plant Leaves, Soybeans.
- physiology : Phakopsora pachyrhizi.
- Mass Spectrometry, Metabolomics.
Abstract
Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of some metabolites in the plant resistance to ASR, which could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen.
DOI: 10.1038/s41598-019-56782-4
PubMed: 31924833
PubMed Central: PMC6954191
Affiliations:
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Oliveira Nunes, Estela" sort="De Oliveira Nunes, Estela" uniqKey="De Oliveira Nunes E" first="Estela" last="De Oliveira Nunes">Estela De Oliveira Nunes</name><affiliation wicri:level="1"><nlm:affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970</wicri:regionArea><wicri:noRegion>86001-970</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Brazilian Agricultural Research Corporation Swine and Poultry, BR-153, Km 110 Distrito de Tamanduá, SC, 89715-899, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Brazilian Agricultural Research Corporation Swine and Poultry, BR-153, Km 110 Distrito de Tamanduá, SC, 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Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080</wicri:regionArea><wicri:noRegion>87020-080</wicri:noRegion></affiliation></author><author><name sortKey="Da Graca, Jose Perez" sort="Da Graca, Jose Perez" uniqKey="Da Graca J" first="José Perez" last="Da Graça">José Perez Da Graça</name><affiliation wicri:level="1"><nlm:affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970</wicri:regionArea><wicri:noRegion>86001-970</wicri:noRegion></affiliation></author><author><name sortKey="Porto, Carla" sort="Porto, Carla" uniqKey="Porto C" first="Carla" last="Porto">Carla Porto</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080</wicri:regionArea><wicri:noRegion>87020-080</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Master in Science, Technology and Food Safety, Cesumar Institute of Science, Technology and Innovation - ICETI, University Center of Maringá - UNICESUMAR, 1610, Guedner Av, Maringá, PR, 87050-900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Master in Science, Technology and Food Safety, Cesumar Institute of Science, Technology and Innovation - ICETI, University Center of Maringá - UNICESUMAR, 1610, Guedner Av, Maringá, PR, 87050-900</wicri:regionArea><wicri:noRegion>87050-900</wicri:noRegion></affiliation></author><author><name sortKey="Martin Do Prado, Rodolpho" sort="Martin Do Prado, Rodolpho" uniqKey="Martin Do Prado R" first="Rodolpho" last="Martin Do Prado">Rodolpho Martin Do Prado</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080</wicri:regionArea><wicri:noRegion>87020-080</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Animal Science, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Animal Science, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080</wicri:regionArea><wicri:noRegion>87020-080</wicri:noRegion></affiliation></author><author><name sortKey="Hoffmann Campo, Clara Beatriz" sort="Hoffmann Campo, Clara Beatriz" uniqKey="Hoffmann Campo C" first="Clara Beatriz" last="Hoffmann-Campo">Clara Beatriz Hoffmann-Campo</name><affiliation wicri:level="1"><nlm:affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970</wicri:regionArea><wicri:noRegion>86001-970</wicri:noRegion></affiliation></author><author><name sortKey="Meyer, Mauricio Conrado" sort="Meyer, Mauricio Conrado" uniqKey="Meyer M" first="Mauricio Conrado" last="Meyer">Mauricio Conrado Meyer</name><affiliation wicri:level="1"><nlm:affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970</wicri:regionArea><wicri:noRegion>86001-970</wicri:noRegion></affiliation></author><author><name sortKey="De Oliveira Nunes, Estela" sort="De Oliveira Nunes, Estela" uniqKey="De Oliveira Nunes E" first="Estela" last="De Oliveira Nunes">Estela De Oliveira Nunes</name><affiliation wicri:level="1"><nlm:affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970</wicri:regionArea><wicri:noRegion>86001-970</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Brazilian Agricultural Research Corporation Swine and Poultry, BR-153, Km 110 Distrito de Tamanduá, SC, 89715-899, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Brazilian Agricultural Research Corporation Swine and Poultry, BR-153, Km 110 Distrito de Tamanduá, SC, 89715-899</wicri:regionArea><wicri:noRegion>89715-899</wicri:noRegion></affiliation></author><author><name sortKey="Pilau, Eduardo Jorge" sort="Pilau, Eduardo Jorge" uniqKey="Pilau E" first="Eduardo Jorge" last="Pilau">Eduardo Jorge Pilau</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil. ejpilau@uem.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080</wicri:regionArea><wicri:noRegion>87020-080</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Mass Spectrometry (MeSH)</term><term>Metabolomics (MeSH)</term><term>Phakopsora pachyrhizi (physiology)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (microbiology)</term><term>Soybeans (metabolism)</term><term>Soybeans (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Feuilles de plante (microbiologie)</term><term>Feuilles de plante (métabolisme)</term><term>Maladies des plantes (microbiologie)</term><term>Métabolomique (MeSH)</term><term>Phakopsora pachyrhizi (physiologie)</term><term>Soja (microbiologie)</term><term>Soja (métabolisme)</term><term>Spectrométrie de masse (MeSH)</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Soja</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Soja</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phakopsora pachyrhizi</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phakopsora pachyrhizi</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Mass Spectrometry</term><term>Metabolomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Métabolomique</term><term>Spectrométrie de masse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of some metabolites in the plant resistance to ASR, which could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31924833</PMID><DateCompleted><Year>2020</Year><Month>11</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>12</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>01</Month><Day>10</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Unraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach.</ArticleTitle><Pagination><MedlinePgn>138</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-019-56782-4</ELocationID><Abstract><AbstractText>Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of some metabolites in the plant resistance to ASR, which could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Evandro</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>da Graça</LastName><ForeName>José Perez</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Porto</LastName><ForeName>Carla</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Master in Science, Technology and Food Safety, Cesumar Institute of Science, Technology and Innovation - ICETI, University Center of Maringá - UNICESUMAR, 1610, Guedner Av, Maringá, PR, 87050-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martin do Prado</LastName><ForeName>Rodolpho</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Animal Science, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hoffmann-Campo</LastName><ForeName>Clara Beatriz</ForeName><Initials>CB</Initials><AffiliationInfo><Affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meyer</LastName><ForeName>Mauricio Conrado</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Oliveira Nunes</LastName><ForeName>Estela</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Brazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina, PR, 86001-970, Brazil.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Brazilian Agricultural Research Corporation Swine and Poultry, BR-153, Km 110 Distrito de Tamanduá, SC, 89715-899, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pilau</LastName><ForeName>Eduardo Jorge</ForeName><Initials>EJ</Initials><AffiliationInfo><Affiliation>Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá, PR, 87020-080, Brazil. ejpilau@uem.br.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="Y">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055432" MajorTopicYN="Y">Metabolomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000067563" MajorTopicYN="N">Phakopsora pachyrhizi</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" 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